Deposition
We work closely with EMBL-EBI to improve and streamline the deposition of structural data into the international archives PDB, EMDB and EMPIAR. CCP-EM v2 contains a task that generates an upload file for EMPIAR, using job tracking within the Doppio project to collate intermediate datasets relevant to the final reconstructions. Similar tools for OneDep deposition to the PDB and EMDB are in preparation.
DRI-IMB
Funded by the UK Digital Research Infrastructure, CCP-EM is collaborating with CCPBioSim, CCP4 and CCPN to develop tools for integrative structural biology. A two-year project is being led by the University of Sheffield to improve data exchange between disciplines and enable joint refinement of integrative models.
In Situ Tomography
The dream of many studies is to understand the structure and dynamics of biomolecules in their native environment. While several pipelines exist for subtomogram averaging of molecules extracted from tomograms, improvements are required for in situ studies to address low signal-to-noise, crowding, low copy number and particle identification. We are working with several groups to improve methods, and make these available in user software.
Ada Lovelace Centre
The STFC Ada Lovelace Centre (ALC) provides computational support to the large experimental facilities on the Harwell campus, including the national cryoEM facility at eBIC. The CCP-EM team work with the ALC to improve the computational processing available at the microscope. New algorithms and software are included in the CCP-EM software suite for wider use.
VolumeEM
VolumeEM is a group of techniques used to image biological material through depths of at least one micrometre at nanometre resolution. A grassroots community has built up over the last 5 years. Through funding from the Digital Research Infrastructure and CoSeC, a consortium is developing the remit of a new Collaborative Computational Project (CCP-volumeEM) across community building, technical scoping, and skills development. CCP-EM is providing advice and support.