April 27th – May 1st | Online
CCP-EM are pleased to confirm that the 2020 CCP-EM Spring Symposium will be held online in a virtual format due to the COVID-19 situation. The conference will be hosted on Zoom and is free of charge, but registration is required – see below.
The conference aims to provide a forum to highlight state of the art developments in computational cryoEM and related themes as well as showcasing outstanding recent applications. We aim to promote an inclusive, friendly atmosphere welcoming both old and new to the community. Also included is the Diamond Light Source Biological Cryo-imaging User Meeting (eBIC & B24).
Topics include instrument technology, sample preparation, image processing, single particle reconstruction, tomography and model building.
Scientific organisers:
Helen Saibil (Birkbeck)
Christos Savva (University of Leicester)
Schedule
Full schedule. Note that all times are in UK local time (BST)
Recordings of most of the talks are available on YouTube.
Monday 27th April: DLS Biological Cryo-imaging User Meeting
- Martin Walsh: Welcome
- Yun Song: eBIC: Update and future plans
- Chidinma Okolo: B24: Update and future plans
- Vicki Gold: A new twist on type IV pili – cryoEM reveals two distinct filaments assembled by the same bacterium
- Ed Morris: CryoEM and Mass Spectrometry analysis of the structural basis of cullin 2-RING E3 Ligase regulation by the COP9 signalosome
- Stefan Balint: T cell derived extracellular particles by Cryo-XRT
- Elizabeth Bolitho: Cryo-XRT of metallodrugs in prostate cancer cells
- Cryo-imaging user meeting discussion
Tuesday 28th April
What’s new in CCP-EM, eBIC and EMDB/EMPIAR?
- Agnel Joseph: CCP-EM
- Yuriy Chaban: eBIC
- Ardan Patwardhan: EMDB/EMPIAR
Keynote presentation
- Chris Russo: A purpose built single-particle electron cryomicroscope: what is needed?
Wednesday 29th April
- Andrea Thorn: Finding secondary structure in CryoEM maps: HARUSPEX
- Morgan Beeby: Evolution of bacterial flagellar motors studied by electron cryotomography
- Hongyi Xu: 3DED/MicroED – Electron Diffraction method for crystal structure determination of biomolecules
- Alex Noble: Neural network particle picking and denoising with TOPAZ
- Adam Simpkin: MrBUMP: Redeployment of automated search model generation for map fitting in CryoEM
Thursday 30th April
- Christopher Aylett: LAFTER and SIDESPLITTER – approaches for handling conformational flexibility and overfitting
- Rouslan Efremov: High-throughput single particle data collection on cryoARM300
- Joe Atherton: CryoEM of Kinesin binding protein: challenges and opportunities from protein-surface interactions
- Jose Miguel de la Rosa Trevin: New developments in Scipion 3.0: consolidating the project as a collaborative software framework
- Miroslava Schaffer: Cryo-FIB lift-out preparation for cryo-electron tomography
Friday 1st May
- Jan Rheinberger: Optimized data acquisition pipeline through ice thickness determination
- Bertram Daum: Investigating archaeal surface structures by CryoEM
- Luiza Mendonça: Subtomogram averaging of the dynamic HIV-1 SP1 6-helix bundle using emClarity
- Dmitry Tegunov: New tools for high-resolution in situ cryo-electron tomography
- Gaia Pigino: Towards a mechanistic understanding of ciliary assembly by CLEM and Cryo-Electron Tomography