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CCP-EM software suite v1.0
CCP-EM are pleased to announce the release of version 1.0 of the CCP-EM software suite, a package containing tools for cryo-EM.
For details of the contents and functionality of the suite, see Recent developments in the CCP-EM software suite (T. Burnley, C.M. Palmer and M.D. Winn, Acta Cryst. D73, 469 - 477, 2017).
To report bugs or give feedback please contact Tom Burnley / Colin Palmer at: firstname.lastname@example.org
CCP-EM v1.0.0 Linux (updated 9 April 2018)
CCP-EM v1.0.0 Mac (updated 9 April 2018)
XQuartz is required for Mac install
Latest nightly builds
CCP-EM 20180427 Linux (updated 27 April 2018)
CCP-EM uses programs from CCP4 for a number of tasks. To use those tasks, please install CCP4 from http://www.ccp4.ac.uk/download/ and activate it before running CCP-EM.
FlexEM and some other programs require Modeller to be installed and for users to have obtained a valid licence key. Please register for a Modeller licence key (free for academic use).
Linux: After running ./install_ccpem.sh you will be prompted to run ./install_modeller.sh. Please run this and enter the license key when prompted.
Mac: Please download and install the Modeller Mac package and use default installation location.
MpUL-multi: Software for Calculation of Amyloid Fibril Mass per Unit Length
from TB-TEM Images.
MpUL-multi is a program for calculating the mass per unit length (MPL) of amyloid fibrils from dark field imaging, by comparing to a standard of known MPL. A graphical user interface allows for simple designation of fibrils and standards. A second program averages intensities from multiple TMV molecules for accurate standard determination, makes multiple measurements along a given fibril, and calculates the MPL. The software is written in Python, and requires a local installation of FIJI or ImageJ. Download and unpack this file, and follow the instructions in README.txt.
A paper describing the approach has been submitted.
TVIPS tools for electron diffraction
The tiff2smv and tvips2smv utilities convert sequences of related electron diffraction images, or sweeps, recorded by Tietz Video and Image Processing Systems (TVIPS) cameras to the Super Marty View (SMV) format. Output SMV images are directly suitable for further processing in suites such as MOSFLM or XDS. tiff2smv supports TIFF images written by either EMMENU or EM_Player, as long as their pixel intensities can be losslessly represented as 16-bit unsigned integers. tvips2smv supports all TVIPS set files written by EMMENU.
Availability: The software is copyright Howard Hughes Medical Institute, and made available under a permissive Internet Software Consortium license.
Johan Hattne, Tamir Gonen (Janelia Research Campus)
Shape Matching for Structural Biology (SMaSB)
The SMaSB software package has been developed to perform volume matching between a query volume and another volume or set of volumes. The primary aim is to search for similar volumes, rather than to optimise a particular fit. The software is primarily a set of Python codes, and has been extensively tested in Ubuntu 12.04 and Mac. More ...
SMaSB - download SMaSB (updated 24/5/16).
This software underpins the PDBeShape service. The authors are Agnel Praveen Joseph, Ingvar Lagerstedt, Ardan Patwardhan and Martyn Winn, with invaluable help from Irene Farabella and Maya Topf. Part of EU-funded BioMedBridges.
Software for Particle Picking - Alan Roseman
The following software is written by Alan Roseman's group at the University of Manchester, UK. The software is distributed under GPLv3. If you download/use the software, please send the author a quick email - improvements to the software depend on future funding which depends on measureable impact!
Software for 3D reconstruction of tip complexes - Ariel Blocker
MRC Image Processing System - Tony Crowther, Richard Henderson & Jude Short (Smith)
Installation is described in /image2010/README. First build all the libraries and executables using a .bld file specific to your system. Binaries will then be written to /image2010/bin and all have the extension .exe